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Summary of protein expression. Constructs used for bacterial expression of KMO exhibited a variety of expression parameters.
| Protein construct | Expressed | Soluble | Active | Yield |
|---|---|---|---|---|
| Full length KMO-6His | Yes | No | Yes | nd |
| Truncate KMO | Yes | Yes | No | nd |
| Full length KMO-12His-FLAG | Yes | Yes | Yes | 19% |
nd, not determined.
