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. 2014 Mar 28;9(3):e93275. doi: 10.1371/journal.pone.0093275

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© 2014 Xu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) HYG marker was used in the DNA detection. In addition, CAPS marker was used to confirm the positive transformants precisely and the restriction enzyme cutting site (BamHI) lied in the premature stopped position of Pid3-J1; (B) Pid3D marker was used in the RNA detection, and Actin is as the referring primer marker.