Figure 5. R539W is sensitive to PKA.

A, representative permeabilized-patch current recordings of WT or R539W channels (co-transfected with yotiao) measured during the voltage-clamp protocol shown. B, mean time course of channel activation by 400 µM cpt-cAMP, 10 µM forskolin and 0.2 µM okadaic acid (cAMP) for WT or R539W KCNE1-KCNQ1 concatemer channel tail currents measured at −40 mV after a 1 s depolarization to +80 mV and normalized to the current value before cAMP application (n = 12–13).