Table 2. Stability measures of the reference genes that were used in RT-qPCR analysis.
All 381 GEO sets | MeSH A11.251 (cells,cultured) | MeSH C04 (Neoplasms) | |||||||
Percentile Rank | Percentile Rank | Percentile Rank | |||||||
Gene Symbol * | Ratio | Mean | Median | Ratio | Mean | Median | Ratio | Mean | Median |
RPS3A | 1 | 96.1 | 99 | 1 | 94.7 | 99 | 1 | 97.3 | 99 |
RPL30 | 1 | 94.4 | 99 | 1 | 95.6 | 99 | 1 | 98.7 | 99 |
RPS10 | 1 | 96.5 | 99 | 1 | 95.4 | 99 | 1 | 98.2 | 99 |
RPL7 | 1 | 96.1 | 99 | 1 | 95 | 99 | 1 | 98 | 99 |
RPL41 | 0.9 | 93.8 | 99 | 0.9 | 92.2 | 99 | 0.9 | 96.7 | 99 |
CFL1 | 1 | 94.9 | 99 | 1 | 93.7 | 99 | 1 | 97.8 | 99 |
RPS17 | 1 | 96.3 | 99 | 1 | 94.7 | 99 | 1 | 97.7 | 99 |
H2AFZ | 0.9 | 90.7 | 94 | 1 | 92.4 | 86 | 0.9 | 93.5 | 95 |
ACTB | 0.9 | 95 | 99 | 1 | 95.3 | 99 | 1 | 98.7 | 99 |
HBXIP | 1 | 89 | 92 | 1 | 88.7 | 92.5 | 1 | 90.4 | 92 |
EEF2 | 1 | 95.2 | 98 | 1 | 93.5 | 98.5 | 1 | 97 | 98.5 |
AARS | 0.9 | 85.7 | 91 | 0.9 | 87.2 | 92 | 0.9 | 87.2 | 92 |
SOD1 | 1 | 93.6 | 97 | 1 | 92.2 | 97 | 1 | 95 | 97 |
TPT1 | 1 | 96.1 | 99 | 1 | 95.3 | 99 | 1 | 97.7 | 99 |
RPN2 | 1 | 91.5 | 95 | 1 | 91.4 | 96 | 1 | 94 | 96.7 |
GSTO1 | 0.9 | 90 | 94 | 0.9 | 89.4 | 94 | 0.9 | 90 | 93 |
*Selected genes with CV ≤0.12 and PO ≥75%. The complete results for all MeSH categories and all reference genes obtained from 381 GEO sets are given in the Supporting Reference Gene Lists dataset (Supporting Information S1).