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. 2014 Mar 28;9(3):e92929. doi: 10.1371/journal.pone.0092929

Figure 4. TEM analysis of compound 1294 treated and untreated N. caninum-infected HFF cultures at 3 days post-infection.

Figure 4

A shows a HFF cell with inhibitor 1294 (2. 5 μM) added at the time point of infection, B represents a higher magnification view of A. Note the absence of parasites, and the fact that compound 1294 does not induce any obvious ultrastructural alterations within the host cell. C and D show N. caninum-infected HFF cultured in the absence of the inhibitor at 3 days post-invasion. Numerous tachyzoites are located within parasitophorous vacuoles, surrounded by a parasitophorous vacuole membrane (PVM), and embedded in a matrix of a tubular membrane network (PVTN). Apical parts of tachyzoites are characterized by the conoid (con), micronemes (mic), and rhoptries (rho); dg  =  dense granules, nuc  =  tachyzoite nucleus, hcnuc  =  host cell nucleus. The two arrows in C point towards proliferating tachyzoites undergoing endodyogeny. Bars in A = 5 μm; B = 1 μm; C = 0.58 μm; D = 0.28 μm.