Figure 7. Compound 1294 treatment reduces cerebral parasite load in N. caninum infected mice.

Balb/c mice (8 animals per experimental group), infected intraperitoneally with 5×10⁶ N. caninum-β-gal tachyzoites, were treated with compound 1294 (50 mg/kg in 100 μL honey suspension). Group A (placebo) received honey for 5 days, group B received 1294 treatment starting at 3 days post infection and continuing daily for 5 days; group C received the same as group B but for 10 days. None of the mice exhibited clinical signs at 28 days post-infection, after which the cerebral parasite load was assessed by quantitative real time PCR.