Fig. 3.

Immunocytochemistry at day 5 of culture. At day 5, all cells expressed OTX2 (a) in the nucleus. FOXA2 (A’, A”) merged images with DAPI nuclear stain. FOXA2 was expressed in 89.2 ± 17.4 % of nuclei, but was expressed also in perinuclear areas (see also Tables 1 and 2). b 93.1 ± 11.1 % of cells expressed NURR1. (B’) All cells expressed TH in the cytoplasm in perinuclear areas (inset; higher magnification), but not in distal processes. (B”) Merged images with DAPI nuclear stain. c All cells expressed EN1 in perinuclear areas and at lower intensity also in the nucleus. (d, D’) All cells expressed β-III tubulin. Bar, (a–d; D’), 50 μm