Table 1.

Immunocytochemistry

Epitope	Percent of total ±SEM
Culture day 5
OTX2	100
FOXA2	89.2 ± 17.4
NURR1	93.1 ± 11.1
EN1	0a
β-III tubulin	100
TH	100
Culture day 25
Dopamine	100
VMAT2	100
EN1	100
FOXA2	89.6 ± 19.4
β-III tubulin	100
GIRK2	100
LMX1b	100
NURR1	100
PITX3	91.6 ± 14.6
Synaptophysin	100
TH	100
Nestin	0
5-HT	0

Data expressed as average cell counts from 20 random fields of view per antibody stain; average 3–12 cells per view. In the case of 100 % positive cells, the entire culture was inspected to validate the scores

^aUnspecific staining in the cytoplasm negatively affected imaging of low nuclear stain