Table 2.

qPCR – comparison in vitro differentiated hEPI-NCSC with human substantia nigra

Gene	Expression level
	(Percent average of three HKG)
	hEPI-NCSC DA neurons	Human SN	Percent of SN
β-III tubulin	73.5 ± 0.2	91.0	80.8 ± 0.2
Tyrosine hydroxylase	73.3 ± 0.9	85.0	86.3 ± 1.0
Dopamine decarboxylase	68.6 ± 1.5	75.0	91.5 ± 2.0
Dopamine-β-hydroxylase	0	ND	–
PNMT	71.0 ± 0.7	100.4	70.7 ± 0.7
NURR1	93.3 ± 0.6	96.7	96.5 ± 0.6
PITX3	67.7 ± 0.3	79.7	84.9 ± 0.4
EN1	81.5 ± 1.6	94.4	86.3 ± 1.7
LMX1b	70.1 ± 2.7	80.9	86.6 ± 3.3
VMAT2	70.3 ± 0.4	81.8	85.7 ± 3.3
GIRK2	63.7 ± 0.7	85.2	72.7 ± 0.2
FOXA2	65.6 ± 1.4	84.4	72.1 ± 1.7
DAT	63.4 ± 1.0	73.5	86.3 ± 1.4

HKG house keeping genes, SN adult human substantia nigra, ND not detected