ROS production by human monocytes controls Leishmania
braziliensis infection. Human monocytes were infected with amastigotes of L. braziliensis (2:1) and treated with NAC or were left untreated. A, Cytospins of cultures were performed at 2 and 72 hours and assessed for the percentage of infected cells and the number of amastigotes per 100 cells using light microscopy. Data from 5 donors from experiments performed independently are shown. B, Light microscopy pictures of representative slides stained with H&E from CTR and NAC treated cultures. C, Parasite titration from cultures of human monocytes treated or not with NAC. Data from 8 donors from independent experiments are shown. *P ≤ .05; **P ≤ .01. Abbreviations: H&E, hematoxylin and eosin; NAC, N-acetylcysteine; ROS, reactive oxygen species.