Fig E2.

Effect of tiotropium on inhibition of capsaicin responses on isolated rat and mouse vagal tissue. The vagal tissue was challenged twice with capsaicin (1 μmol/L) to evoke 2 consecutive control depolarization responses, and then the tissue was exposed to vehicle or tiotropium (1 nmol/L) for 30 minutes. The tissue was then exposed to capsaicin in the presence of tiotropium. After a washout, the tissue was rechallenged with capsaicin to confirm tissue viability. A, Inhibition of depolarization induced by capsaicin (1 μmol/L) in rat tissue. B, An example trace. C, Depolarization (mV) of mouse isolated vagus nerve tissue induced by capsaicin or capsaicin in the presence of tiotropium and recovery capsaicin response, indicating that there is no inhibition of the response in mouse tissue. D, An example trace in mouse tissue.