Fig E5.

Effect of tiotropium on capsaicin responses in a FLIPR assay (work performed by using GenScript). HEK293 cells were genetically engineered to overexpress human TRPV1, plated at 20,000 cells per well, and stimulated with capsaicin. A, Concentration response curve with data expressed as relative fluorescence units (R.F.U.). B-D, From these data, a submaximal concentration of capsaicin was chosen to profile the positive control, TRPV1 antagonist JNJ-172030212 (Fig 5, B), tiotropium (Fig 5, C), and glycopyrrolate (Fig 5, D). Experiments were run in duplicates.