Fig. 3.

i⁶A treatment of MCF7 cells induced the NRF2 pathway. MCF7 cells were transiently transfected with a reporter gene plasmid in which the firefly luciferase gene was under the control of a minimal CMV promoter containing multiple antioxidant response elements (AREs). After 24 h, cells were treated with 10 µM i⁶A for 6 h or left untreated. Firefly luciferase activity was normalized to that of Renilla luciferase expressed constitutively from a control plasmid, to control for transfection efficiency. Values are mean and SE of six independent transfections. ***P < 0.0001 versus untreated cells.