Fig. 3.

(A) Normalized absorbance and fluorescence spectra of NBD-H (200 µmol/l) after reaction with MG (100 µmol/l) in 100 mM phosphate buffer pH 7.4. (B) Time-course of the appearance of the fluorescence (ex. 500 nm; em. 560 nm) at 37 °C of NBD-H (200 µM) with MG (♦) and HNE (■) at 100 µM. (C) Fluorescence response of MG (♦) and HNE (■) after 10 min incubation at 37 °C with NBD-H with increasing amounts of carbonyls (0.05–20 mM).