Fig. 6.

Inhibition of AGE-BSA formation by various compounds. BSA (50 mg/ml) was incubated at 37 °C for 24 h in 0.4 M phosphate buffer, pH 7.4 in the absence and presence of various concentrations of the compounds with (A) 5 mM methylglyoxal, (B) 5 mM 4-Hydroxynonenal, (C) 20 mM glucose. IC₅₀ values of inhibition of AGE-BSA formation are expressed in equivalent of aminoguanidine. Each data point is the mean±SD from triplicate determinations. In addition, one-way ANOVA of the values yielded p<0.01. *p>0.05 versus controls in the absence of scavenger.