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. Author manuscript; available in PMC: 2014 Mar 29.
Published in final edited form as: Vet Comp Oncol. 2011 Dec 8;11(2):124–139. doi: 10.1111/j.1476-5829.2011.00309.x

Figure 4.

Figure 4

Met, EGFR and Ron co-associate in canine OSA. Met, EGFR or Ron was immunoprecipitated from canine OSA cell lines followed by western blot analysis for Met, EGFR or Ron to determine interactions between these RTKs. (A and B) Met and EGFR interaction occurred in canine OSA cell lines and (C) primary canine OSA tumours. T1 = canine primary OSA tumour 1; T2 = canine primary OSA tumour 2 (A) Met was associated with Ron in canine OSA cell lines. Western blot analysis was performed simultaneously (A and B right side) to confirm protein expression of EGFR and Ron in the cell lines. [Note: immunoprecipitation (IP) is usually required for Met.]