Figure 3.

MDA-MB-231 cells with different features characteristic of apoptosis and secondary necrosis as detected by dual staining with AO and PI after 72 hours treatment with EA extract of C. calcitrans. Apoptotic cells showed membrane blebbing, DNA fragmentation and chromatin condensation. Necrotic cells showed diffused chromatin with apparent nucleus membrane disruption. (a) Untreated control; (b) Low concentration (30 ug/mL); (c) IC50 (60 ug/mL); (d) High concentration (120 ug/mL) (magnification: ×400)