Table 2.
Frequency of phenotypic marker immunohistochemical scores for NP cell-laden hydrogels of varying formulations.
| N-cadherin | ||||||
|---|---|---|---|---|---|---|
|
| ||||||
| score | 5% PEG | 10% PEG | 5% PEG | 10% PEG | 5% PEGa | 10% PEG |
| 0 μg/ml LM111 | 100 μg/ml LM111 | 500 μg/ml LM111 | ||||
| 0 | 4 | 0 | 6 | 4 | 0 | 6 |
| 1 | 5 | 8 | 1 | 4 | 1 | 3 |
| 2 | 0 | 1 | 2 | 0 | 4 | 0 |
| 3 | 0 | 0 | 0 | 0 | 4 | 0 |
| cytokeratin 8 | ||||||
|
| ||||||
| score | 5% PEG | 10% PEG | 5% PEGb | 10% PEG | 5% PEGb | 10% PEG |
| 0 μg/ml LM111 | 100 μg/ml LM111 | 500 μg/ml LM111 | ||||
|
| ||||||
| 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1 | 7 | 6 | 1 | 6 | 0 | 8 |
| 2 | 2 | 3 | 2 | 3 | 4 | 1 |
| 3 | 0 | 0 | 6 | 0 | 5 | 0 |
| integrin α3 | ||||||
|
| ||||||
| score | 5% PEG | 10% PEG | 5% PEGc | 10% PEG | 5% PEGd | 10% PEG |
| 0 μg/ml LM111 | 100 μg/ml LM111 | 500 μg/ml LM111 | ||||
|
| ||||||
| 0 | 1 | 0 | 0 | 0 | 0 | 0 |
| 1 | 5 | 7 | 0 | 4 | 1 | 4 |
| 2 | 0 | 2 | 1 | 3 | 4 | 5 |
| 3 | 0 | 0 | 8 | 2 | 4 | 0 |
Significantly different from hydrogels containing 5% PEG (0 or 100 μg/ml LM111) and formulations containing 10% PEG (0, 100 or 500 μg/ml) (p<0.004)
Significantly different from formulations containing 10% PEG (0, 100 or 500 μg/ml LM111) and hydrogels containing 5% PEG (0 μg/ml LM111) (p<0.004)
Significantly different from formulations containing 5% PEG (0 μg/ml LM111) and 10% PEG (0 or 500 μg/ml LM111) (p<0.004)
Significantly different from formulations containing 5% (0 μg/ml LM111) or 10% PEG (0 μg/ml LM111) (p<0.004)