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. Author manuscript; available in PMC: 2015 Feb 10.
Published in final edited form as: Chembiochem. 2014 Jan 29;15(3):435–442. doi: 10.1002/cbic.201300701

Table 2.

Bacterial strains and plasmids used in this study.

Strain or plasmid Relevant properties Source or
reference
Strains
  P. aeruginosa
    PAO-JP2 PAO1 lasI::Tet rhlI::Tn501-2; HgR TcR [33]
    PAO-JG21 PAO-JP2 Δ(mexA-mexB-oprM) This study
  E. coli
    DH5α E. coli strain for transformation [34]
    S17-1 Mobilizer strain [35]
    JLD271 K-12 ΔlacX74 sdiA271::Cam; ClR [36]
Plasmids
  pEX18Gm GmR; oriT+ sacB+, gene replacement
vector with MCS from pUC18; pEX100T
backbone GmR 		[37]
  pJG034 pEX18Gm with markerless Δ(mexAB-
oprM)		 This study
  plasI-LVAgfp lasI’-gfp[LVA] transcriptional fusion; CbR [38]
  pSC11 Broad host range lasI-lacZ reporter;
source of lasI DNA; ApR 		[39]
  pPROBE-KT Broad host range promotorless gfp
transcriptional fusion vector; KmR 		[40]
  pPROBE-KL lasI’-gfp[LVA] transcriptional fusion; KmR This study
  pJN105L Arabinose-inducible expression vector
for lasR; pBBRMCS backbone; GmR 		[41]