Table 2.
Strain or plasmid | Relevant properties | Source or reference |
---|---|---|
Strains | ||
P. aeruginosa | ||
PAO-JP2 | PAO1 lasI::Tet rhlI::Tn501-2; HgR TcR | [33] |
PAO-JG21 | PAO-JP2 Δ(mexA-mexB-oprM) | This study |
E. coli | ||
DH5α | E. coli strain for transformation | [34] |
S17-1 | Mobilizer strain | [35] |
JLD271 | K-12 ΔlacX74 sdiA271::Cam; ClR | [36] |
Plasmids | ||
pEX18Gm | GmR; oriT+
sacB+, gene replacement vector with MCS from pUC18; pEX100T backbone GmR |
[37] |
pJG034 | pEX18Gm with markerless Δ(mexAB- oprM) |
This study |
plasI-LVAgfp | lasI’-gfp[LVA] transcriptional fusion; CbR | [38] |
pSC11 | Broad host range lasI-lacZ reporter; source of lasI DNA; ApR |
[39] |
pPROBE-KT | Broad host range promotorless gfp transcriptional fusion vector; KmR |
[40] |
pPROBE-KL | lasI’-gfp[LVA] transcriptional fusion; KmR | This study |
pJN105L | Arabinose-inducible expression vector for lasR; pBBRMCS backbone; GmR |
[41] |