Table 2.
hb1-1 Depletion by RNAi Suppresses the Phenotype of Extra Seam Cells and Retarded Alae of a mir-48 mir-241; mir-84 Triple Mutant and of a lin-46 mir-48 mir-241; mir-84 Mutant
| Strain | Genotype | dsRNA Construct | Number of Seam Cells (n)
|
Percentage of Worms with Precocious Alae during L4
|
Percentage of Worms with Alae at L4 Molt/Young Adult
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| L3 | L4 | No Alae | Gapped | Complete | n | No Alae | Gapped | Complete | n | |||
| N2 | wild-type | control | 11 (15) | 11 (20) | 100 | 0 | 0 | 20 | 0 | 0 | 100 | 26 |
| hbl-1 | 9 (33) | 10 (33) | 0 | 24 | 76 | 33 | 0 | 14 | 86 | 7 | ||
| lin-41 | 11 (16) | 11 (40) | 60 | 40 | 0 | 40 | 0 | 29 | 71 | 24 | ||
| hbl-1 and lin-41 | 10 (27) | 11 (35) | 3 | 57 | 40 | 35 | 0 | 46 | 54 | 13 | ||
| VT1066 | mir-48 mir-241; mir-84 | control | 17 (21) | 19 (23) | 100 | 0 | 0 | 23 | 43 | 54 | 4 | 28 |
| hbl-1 | 9 (33) | 14 (48) | 89 | 11 | 0 | 36 | 0 | 32 | 68 | 25 | ||
| lin-41 | 16 (20) | 17 (32) | 93 | 7 | 0 | 29 | 0 | 47 | 53 | 30 | ||
| hbl-1 and lin-41 | 10 (20) | 11 (30) | 0 | 60 | 40 | 25 | 0 | 29 | 71 | 21 | ||
| VT786 | lin-46 | control | 12 (9) | 12 (20) | 100 | 0 | 0 | 20 | 0 | 19 | 81 | 26 |
| hbl-1 | 9 (6) | 10 (20) | 0 | 20 | 80 | 20 | 0 | 0 | 100 | 15 | ||
| lin-41 | 12 (1) | 12 (21) | 81 | 19 | 0 | 21 | 0 | 12 | 88 | 17 | ||
| hbl-1 and lin-41 | 8 (3) | 10 (20) | 11 | 37 | 53 | 19 | 0 | 8 | 92 | 24 | ||
| VT1145 | lin-46 mir-48 mir-241; mir-84 | control | 22 (17) | 40 (20) | 100 | 0 | 0 | 20 | 100 | 0 | 0 | 18 |
| hbl-1 | 10 (24) | 14 (36) | 100 | 0 | 0 | 36 | 0 | 80 | 20 | 20 | ||
| lin-41 | 22 (20) | 40 (31) | 100 | 0 | 0 | 31 | 100 | 0 | 0 | 36 | ||
| hbl-1 and lin-41 | 11 (22) | 11 (36) | 0 | 50 | 50 | 36 | 0 | 29 | 71 | 17 | ||