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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Cancer. 2014 Jan 10;120(4):579–588. doi: 10.1002/cncr.28555

Figure 2. EWS-FLI-1 regulated REST expression in hMSC, HNP and TC71 cells.

Figure 2

Figure 2

Figure 2

Figure 2

A. hMSC (left panel) and HNP( right panel) cells were transfected with pG5-EWS-FLI-1 or a control plasmid. REST and EWS-FLI-1 expression levels were determined by RT-PCR. GAPDH was used as the internal control.

B. hMSC were transfected with pG5-EWS-FLI-1 or a control plasmid. REST protein expression was detected by Western blot. β-Actin was used as the internal control.

C. TC71 and A4573 human Ewing sarcoma cells were transfected with siEWS-FLI-1 or siControl vector. EWS and REST protein levels were determined by Western blot. β-Actin was used as the internal control.

D. TC71 cells were transfected with siREST or the siControl vector. EWS-FLI-1 and REST levels were determined by RT-PCR. The 18S primer was used as the internal control.