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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Cancer. 2014 Jan 10;120(4):579–588. doi: 10.1002/cncr.28555

Figure 5. Suppression of REST increased hypoxia and apoptosis in TC71 tumor tissues.

Figure 5

Figure 5

Figure 5

Figure 5

Figure 5

A. Hypoxyprobe (HPI) was injected into mice 150 min before sacrifice. Tumor tissues were analyzed using anti-hypoxyprobe antibody to detect HPI expression (red) in TC71–siREST and TC71–siControl tumors.

B. HPI expression was quantified by microscopy software Simple PCI in at least five random microscopy fields from different samples, and averages were calculated. The difference between the two groups was statistically significant (P<0.01).

C. HPI (green) and desmin (red) double immunofluorescent staining was performed. HPI expression levels were higher and desmin expression levels were lower in TC71–siREST tumor samples than those in TC71–siControl samples.

D. TUNEL assay was performed to detect apoptotic cells (green) in TC71–siREST and TC71-siControl tumor samples.

E. Apoptotic cells were quantified by Simple PCI in at least five random microscopy fields, and group averages were calculated. The difference between the two groups was statistically significant (P<0.01).