Table 4.
Intra‐run Precision of the Tricyclic Antidepressant HPLC/MS/MSAssay
| Mean ± SD ng/ml (%CV); n = 6 | ||||
|---|---|---|---|---|
| LOQ (25 ng/ml) | LQC (75 ng/ml) | MQC (300 ng/ml) | HQC (1,500 ng/ml) | |
| Amitriptyline | 27 ± 2.2 (8) | 83 ± 6.0 (7) | 344 ± 21 (6) | 1,420 ± 170 (12) |
| Clomipramine | 25 ± 2.3 (9) | 82 ± 5.7 (7) | 310 ± 12 (4) | 1,620 ± 130 (8) |
| Cyclobenzaprine | 26 ± 2.6 (10) | 85 ± 4.2 (5) | 276 ± 27 (10) | 1,600 ± 64 (4) |
| Desipramine | 25 ± 3.7 (15) | 78 ± 2.3 (3) | 331 ± 26 (8) | 1,400 ± 182 (13) |
| Doxepin | 23 ± 1.4 (6) | 85 ± 4.3 (5) | 276 ± 27 (10) | 1,594 ± 48 (3) |
| Fluoxetine | 27 ± 2.7 (10) | 79 ± 8.7 (11) | 297 ± 33 (11) | 1,440 ± 173 (12) |
| Imipramine | 26 ± 1.8 (7) | 80 ± 11 (14) | 312 ± 25 (8) | 1,995 ± 180 (9) |
| Maprotiline | 26 ± 1.3 (5) | 83 ± 5.0 (6) | 267 ± 16 (6) | 1,327 ± 106 (8) |
| Mirtazapine | 25 ± 4.5 (18) | 80 + 8.0 (10) | 305 ± 21 (7) | 1,648 ± 132 (8) |
| Norclomipramine | 28 ± 0.8 (3) | 82 ± 5.0 (8) | 307 ± 12 (4) | 1,581 ± 237 (15) |
| Nordoxepin | 26 ± 3.9 (15) | 72 ± 10 (15) | 283 ± 42 (15) | 1,251 ± 25 (2) |
| Norfluoxetine | 25 ± 3.0 (12) | 73 ± 8.0 (11) | 310 ± 37 (12) | 1,503 ± 150 (10) |
| Nortriptyline | 27 ± 1.9 (7) | 85 ± 1.7 (2) | 325 ± 23 (7) | 1,532 ± 153 (10) |
| Trazodone | 23 ± 2.1 (9) | 67 + 4.0 (6) | 238 ± 33 (14) | 1,681 ± 168 (10) |
| Trimipramine | 26 ± 2.8 (11) | 86 ± 4.3 (5) | 294 ± 24 (8) | 1,398 ± 154 (11) |