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. Author manuscript; available in PMC: 2014 Mar 30.
Published in final edited form as: Gene. 2012 Oct 2;511(2):143–150. doi: 10.1016/j.gene.2012.09.093

Figure 3.

Figure 3

Disruption of aincar morphogenesis by overexpression of miR-21. A) The RNA levels of miR-21 were measured in mK-ras-LE-miR-21 and mK-ras-LE-vec variants using qRT-PCR. A fold increase in miR-21 was determined by setting the values from mK-ras-LE-vec to one. B) Total cell lysates were extracted from mK-ras-LE-miR-21 and mK-ras-LE-vec variants in rBM 3-D culture. The protein levels of PTEN were determined using immunoblots. C) mK-ras-LE-miR-21 and mK-ras-LE-vec variants were monitored in rBM 3-D culture for 12 days. Morphogenesis of the mK-ras-LE variants in rBM 3-D culture was recorded using an inverse phase contrast microscope. Representative cell colonies were pointed at by white arrows.