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Primers and Probes used in Taq-Man Assays
| Primer and Probe Sequences | |
|---|---|
| HpolF | GAAATTTGTACAGAAATGGAAAAGGAA |
| HpolR | TGAGTTCTCTTATTAAGTTCTCTGAAATCTAC |
| HpolProbe | 6FAM—TTGGGCCTGAAATCCATACAATACTCCAGT—TAMRA |
| IC-F | GCTCGCAGAAATCGTTGGA |
| IC-R | CAGCGAGTGGTTCGACAATTAC |
| IC-Probe | VIC—CCGCTAGGGTCCAAC—TAMRA |
Primer and probe sets were designed using Primer Express software, (version 1.0, Applied Biosystems, Foster City, CA), and obtained via custom order from Applied Biosystems.
