Table 3.
Input Amount | % Detection |
---|---|
1 copy | 56%* |
3 copies | 73% |
5 copies | 97% |
10 copies | 100% |
Hpol RNA templates were diluted to the estimated copy number and detection frequencies determined from analysis of 6 replicates at each dilution in 5 independent quantitation assays (total of 30 replicates per dilution).
55.6% detection fits expected Poisson probability of one copy detection rate (22); observed versus expected rates of detection were not statistically different (Yates-corrected X2 1.461, p > 0.20)