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. Author manuscript; available in PMC: 2014 Mar 30.
Published in final edited form as: Cytometry A. 2009 Dec;75(12):979–989. doi: 10.1002/cyto.a.20814

Figure 2.

Figure 2

Data collected on 8-peak rainbow microspheres using the demonstration system on a slow flow flow cytometer. A. A bivariate histogram of Side Scatter Peak versus area, which was used to gate the fluorescence data. B. Fluorescence area histogram from the gate shown in A. C. A linear fit of the means of the populations shown in B as a function of the calibrated MESF-PE values of the microspheres. The fit was that was weighted by the inverse of square of the standard deviation of each point. Error bars shown are two standard deviations of the mean fluorescence area point. The resultant fit gave a slope of 0.044 +/− 0.0007 mean fluorescence channels per MESF-PE, an intercept of 12.3 +/− 2.8 mean fluorescence area channels, and a R2 of 0.999. The data has presented with breaks in the axes to display the data with greater visual clarity.