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. Author manuscript; available in PMC: 2014 Mar 30.
Published in final edited form as: Cell. 2013 May 2;153(4):910–918. doi: 10.1016/j.cell.2013.04.025

Figure 2. Single- and Double-Gene Targeting In Vivo by Injection into Fertilized Eggs.

Figure 2

(A) Genotyping of Tet1 single-targeted mice.

(B) Upper: genotyping of Tet2 single-targeted mice. RFLP analysis; lower: Southern blot analysis.

(C) The sequence of both alleles of targeted gene in Tet1 biallelic mutant mouse 2 and Tet2 biallelic mutant mouse 4.

(D) Genotyping of Tet1/Tet2 double-mutant mice. Analysis of mice 1 to 12 is shown. Upper: RFLP analysis; lower: southern blot analysis. The Tet1 locus is displayed on the left and the Tet2 locus on the right.

(E) The sequence of four mutant alleles from double-mutant mouse 9 and 10. PAM sequences are labeled in red.

(F) Three-week-old double-mutant mice. All RFLP and Southern digestions and probes are the same as those used in Figure 1. See also Figures S2 and S3.