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. Author manuscript; available in PMC: 2014 Mar 30.
Published in final edited form as: Cell. 2013 May 2;153(4):910–918. doi: 10.1016/j.cell.2013.04.025

Figure 3. Multiplexed HDR-Mediated Genome Editing In Vivo.

Figure 3

(A) Schematic of the oligo-targeting sites at Tet1 and Tet2 loci. The sgRNA-targeting sequence is underlined, and the PAM sequence is labeled in green. Oligo targeting each gene is shown under the target site, with 2 bp changes labeled in red. Restriction enzyme sites used for RFLP analysis are bold and capitalized.

(B) RFLP analysis of double oligo injection mice with HDR-mediated targeting at the Tet1 and Tet2 loci.

(C) The sequences of both alleles of Tet1 and Tet2 in mouse 5 and 7 show simultaneously HDR-mediated targeting at one allele or two alleles of each gene, and NHEJ-mediated disruption at the other alleles. See also Figure S4.