. Author manuscript; available in PMC: 2014 Mar 30.

Published in final edited form as: Cell. 2013 May 2;153(4):910–918. doi: 10.1016/j.cell.2013.04.025

Table 1. CRISPR/Cas-Mediated Gene Targeting in V6.5 ES Cells.

Gene	Mutant Alleles per Clone/Total Clones Tested
Gene	6	5	4	3	2	1	0
Tet1	N/A				27/48	4/48	17/48
Tet2					37/48	2/48	9/48
Tet3					32/48	3/48	13/48
Tet1+ Tet2 + Tet3	20/96	16/96	2/96	2/96	1/96	0/96	55/96

Plasmids encoding Cas9 and sgRNAs targeting Tet1, Tet2, and Tet3 were transfected separately (single targeting) or in a pool (triple targeting) into ES cells. The number of total alleles mutated in each ES cell clone is listed from 0 to 2 for single-targeting experiment, and 0 to 6 for triple-targeting experiment. The number of clones containing each specific number of mutated alleles is shown in relation to the total number of clones screened in each experiment. See also Table S1.