Table 2. CRISPR/Cas-Mediated Single-Gene Targeting in BDF2 Mice.
| Gene | Cas9/sg RNA (ng/μl) | Blastocysts/Injected Zygotes | Transferred Embryos (Recipients) | Newborns (Dead) | Mutant Alleles per Mouse/Total Mice Testeda | ||
|---|---|---|---|---|---|---|---|
| 2 | 1 | 0 | |||||
| Tet1 | 200/20 | 38/50 | 19 (1) | 2 (0) | 2/2 | 0/2 | 0/2 |
| 100/20 | 50/60 | 25 (1) | 3 (0) | 2/3 | 0/3 | 1/3 | |
| 50/20 | 40/50 | 40 (2) | 8 (3) | 4/7 | 2/7 | 1/7 | |
| 100/50 | 167/198 | 60 (3) | 12 (2) | 9/11 | 1/11 | 1/11 | |
| Tet2 | 100/50 | 176/203 | 108 (5) | 22 (3) | 19/20 | 0/20 | 1/20 |
| Tet3 | 100/50 | 85/112 | 64 (4) | 15 (13) | 9/13 | 2/13 | 2/13 |
Cas9 mRNA and sgRNAs targeting Tet1, Tet2, or Tet3 were injected into fertilized eggs. The blastocysts derived from injected embryos were transplanted into foster mothers and newborn pups were obtained and genotyped. The number of total alleles mutated in each mouse is listed from 0 to 2. The number of mice containing each specific number of mutated alleles is shown in relation to the total number of mice screened in each experiment. See also Table S2.
a
Some of the pups were cannibalized.