| Component | Amount (μl) |
|---|---|
| FastDigest buffer, 10× | 3 |
| FastDigest EcoRI | 1 |
| FastDigest HindIII | 1 |
| Purified PCR product, 20 ng μl−1 | 10 |
| (Step 78 or 101) | |
| ddH2O | 15 |
| Total volume | 30 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.