| Component | Amount (μl) |
|---|---|
| FastDigest buffer, 10× | 3 |
| FastDigest EcoRI | 1 |
| FastDigest HindIII | 1 |
| FastAP alkaline phosphatase | 1 |
| pUC19 vector (200 ng μl−1) | 5 |
| ddH2O | 20 |
| Total volume | 30 |
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