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. 2014 Apr;184(4):1073–1084. doi: 10.1016/j.ajpath.2013.12.019

Figure 4.

Figure 4

Effect of morphine on leukocyte migration into LCC-derived conditioned media–containing PVA sponge assay in mice. A: Graphs show the fold change in RLU. Luminescence was collected as a measure of Luc+BM infiltrating preimplanted LLC-CM-PVA sponges in WT and MORKO Luc-expressing mice (n = 4 per group) (∗P < 0.25). Graphs show the relative migration of WT Luc+BM cells isolated from placebo- and morphine-treated Luc-positive mice and injected via tail vein into non-Luc MORKO host mice (n = 4 per group) (∗P < 0.05) (B); the relative migration of naive MORKO Luc+BM cells in drug pretreated WT mice (n = 6 per group) (∗P < 0.005) (C); percent change in normalized TER from time on addition of naive human PLB985 cells to vehicle and morphine (1.0 μmol/L) pretreated HUVECs (D); and saline- or morphine-pretreated huPLB985 cells to human TNF-α (100 ng/mL every 2 hours)–pretreated huMVECs (E). LPS induces increase in ICAM-1 on HUVECs as determined using flow cytometry (∗P < 0.05) (F).