Table 3.
Differences in epitope recognition between immunized and infected C57Bl/10 mice.
| Foot pad injection | T-cell proliferation to peptides |
Antibody level |
|||||
|---|---|---|---|---|---|---|---|
| 44–64 | 65–83 | 123–143 | 350–368 | p1–20 | p201–220 | TB71 | |
| Recombinant PstS1a | − | ++ | ++ | − | − | +++ | ++ |
| Heat-killed H37Rva | − | − | + | + | +++ | − | + |
| H37Rv infection | + | + | − | − | − | − | ++ |
Magnitude of the immune response: stimulation indices: −, <1; +, 1–10; ++, 10–20 of spleen and lymph node cells 7 days after immunization.
ELISA peptide binding or TB71 mAb competition titers: –, <10; +, 10–100; ++, 100–1000; +++, >1000 in sera harvested 12 weeks after first inoculation.
aAntigen in incomplete Freund’s adjuvant followed by three boosters without adjuvant. Data from Vordermeier et al. (89).