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. Author manuscript; available in PMC: 2015 Mar 21.
Published in final edited form as: Analyst. 2014 Mar 21;139(6):1355–1363. doi: 10.1039/c3an02400h

Scheme 1.

Scheme 1

Overview of the on-chip extraction/purification of biotinylated membrane proteins from cell lysates using the μSPE device. (A) Micropillar activation and NeutrAvidin immobilization; (B) whole cell lysate infusion where the biotinylated membrane proteins are affinity captured while contaminating cytosolic proteins are eluted; and (C) FITC-avidin addition used to label unreacted biotin of selected membrane proteins followed by disulfide bond reduction releasing either FITC-avidin labeled membrane proteins or unlabeled membrane proteins from the μSPE bed. Symbols are defined in the legend.