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. Author manuscript; available in PMC: 2015 Jan 27.
Published in final edited form as: Dev Cell. 2014 Jan 9;28(2):117–131. doi: 10.1016/j.devcel.2013.11.023

Figure 4. Knocking down AnkG or over-expression of AnkG dominant-negative fragments differentially changes action potential firing by altering inward and outward currents.

Figure 4

A, Effects of knocking down AnkG on the action potential waveform recorded with current clamp. B, Effects of knocking down AnkG on inward (downward) and outward currents recorded with voltage clamp. Neurons were transfected with siRNA plasmids at 5 DIV and recorded at 11 DIV. C, Differential effects of expressing YFP-AR1 and YFP-AR2 on action potential firing recorded with current clamp. Neurons were transfected at 5 DIV and recorded at 7–8 DIV. D, Effects of YFP-AR1 and YFP-AR2 on inward and outward currents recorded with voltage clamp. E, Summary of the effects on AP amplitude (AP in mV). F, Summary of the effects on AP duration (ms). G, Summary of the effects on inward current density (Iin). H, Summary of the effects on outward current density (Iout). The "n" number is indicated in each column in E. An unpaired t-test was used for the siRNA experiments and One-Way ANOVA followed by Dunnett's test was used for the experiments of YFP-AR1 and YFP-AR2. *, p < 0.05; **, p < 0.01; ***, p < 0.001. See also Figure S4.