Fig. 2.

Effects of sulfhydryl reagents on l-lysine oxidation. The oxidase activity followed the hydrogen peroxide was determined as described under experimental procedures. The enzyme, preincubated with varying amounts of p-CMB, was added to the assay mixture containing 40 mM l-lysine and 20 mM potassium phosphate buffer, pH 7.0 (n = 3). Black circles, wild-type l-AAO/MOG; white circles, l-AAO/MOG C254I.