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. Author manuscript; available in PMC: 2014 Mar 31.
Published in final edited form as: J Exp Bot. 2011 Dec 22;63(4):1725–1733. doi: 10.1093/jxb/err403

Fig. 3. Calcium-dependent phosphorylation assays on recombinant PsaN, CAS, VAR1, VAR2, and GST protein.

Fig. 3

The two middle lanes of the left panel display the autoradiograph of 32P-labelled proteins of a Ca2+-dependent phosphorylation assay of recombinant protein (prot) by an Arabidopsis chloroplast protein extract (chloro). The two left lanes are a control of protein only, whereas the two right lanes are a control for the background phosphorylation in the chloroplast protein extract. Note that GST is not being phosphorylated, instead a strong phosphorylation is visible in the EGTA sample of the chloroplast protein extract. The right panel is the protein loading control (CBB=Coomassie Brilliant Blue).