Figure 2.

AnnexinV-FITC/PI double staining was used to quantify apoptosis of differentiated PC12 cells after microwave exposure. The results showed that apoptosis and necrosis of PC12 cells increased obviously at 6 h after 30 mW/cm² microwave exposure (A), and 30 mW/cm² was the lowest effective dose for radiation-induced apoptosis in this study (B). Moreover, the time course of radiation-induced apoptosis showed that 6 h was the peak time of apoptosis after radiation exposure (C). Significant differences are as follows: vs. control, *p < 0.05, **p < 0.01 (ANOVA test). Scale bars: A, 10 µm.