Table 2.
Yields and purities of DNA extracted from FFPE tissues according to eight protocols.
| Extraction Method | Yield (ng) | Concentration (ng/ul) | A260/280 | ||
|---|---|---|---|---|---|
| Mean | SD | Mean | SD | Range | |
| A1 | 1285.75 | 360.76 | 137.69 | 37.56 | 1.76-2.04 |
| B1 | 207.00 | 114.86 | 29.97 | 10.75 | 1.83-2.03 |
| C1 | 1178.25 | 135.92 | 273.05 | 175.44 | 1.06-2.01 |
| D1 | 152.75 | 121.12 | 24.32 | 19.96 | 1.86-2.31 |
| A2 | 115.25 | 359.73 | 259.22 | 82.24 | 1.42-1.97 |
| B2 | 366.75 | 255.56 | 73.73 | 20.23 | 1.67-2.05 |
| C2 | 5088.25 | 2824.14 | 576.69 | 249.24 | 1.94-2.08 |
| D2 | 217.00 | 60.54 | 28.55 | 4.32 | 1.89-2.00 |
SD: Standard deviation
A1: Deparaffinized in Tubes+Digested ON+Using Commercial Kit
B1: Deparaffinized in Tubes+Digested ON+ Using Phenol-chloroform
C1: Deparaffinized in Tubes+Digested 72 hours+Using Commercial Kit
D1: Deparaffinized in Tubes+Digested 72 hours+ Using Phenol-chloroform
A2: Deparaffinized on Slide + Digested ON+Using Commercial Kit
B2: Deparaffinized on Slide + Digested ON+ Using Phenol-chloroform
C2: Deparaffinized on Slide +Digested 72 hours+ Using Commercial Kit
D2: Deparaffinized on Slide +Digested 72 hours+ Using Phenol-chloroform.