Endothelial expression of cad-11 in E18 mice. A-C, Hematoxylin and eosin staining of the aortic valve, pulmonic valve, mitral valve, and tricuspid valve in frontal sections. D, H, Immunofluorescence staining of cad-11 protein (red) and MF20 (green) at low (D) and high (H) magnification of the aortic valve. E, I, Immunofluorescence staining of cad-11 protein (red) and MF20 (green) at low (E) and high (J) magnification of the pulmonic valve. F, J, Immunofluorescence staining of cad-11 protein (red) and MF20 (green) at low (F) and high (J) magnification of the mitral valve. G, K, Immunofluorescence staining of cad-11 protein (red) and MF20 (green) at low (G) and high (K) magnification of the tricuspid valve. White arrows indicate endothelial cells expressing cad-11. Abbreviations: AoV, aortic valve; PV, pulmonic valve; MV, mitral valve; TV, tricuspid valve; LA, left atrium; RA, right atrium; Thy, thymus; IRS, Interventricular septum. Scale bar = (A-C) 1mm, (D-G) 50 μm, (H-K) 20 μm.