Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 28;59(4):1351–1365. doi: 10.1002/hep.26938

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Authors. Hepatology published by Wiley on behalf of the American Association for the Study of Liver Diseases

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Fig 3 — eGFP⁺CD8⁺ T_reg cells are more potent suppressors compared to eGFP⁻CD8⁺ T_reg cells from IL-10 transcriptional reporter mice and display some canonical T_reg markers. C57BL/6 mice were IV infected with 2.5 × 10⁷ IU of Ad-Ova, day 7 liver MNCs were isolated, and total RNA was collected from FACS-sorted Ova Tet⁺ and Ova Tet⁻CD8⁺ T_reg cells. (A) Il10 (IL-10), Il2 (IL-2), Ifng (IFN-γ), Tgfb (TGF-β), Lgals9 (Gal-9), Havcr2 (Tim-3), Pdl1 (PD-L1), Pdc1 (PD-1), Ctla4 (CTLA-4), Klrc1 (NKG2A), Foxp3 (FoxP3), and Ikzf2 (Helios) mRNA was measured by qPCR. (B) Expression of intranuclear/cellular Tbet/Gata-3/FoxP3/Helios and surface CD25/CTLA-4/NKG2A/GITR was determined on CD8⁺ T_reg cells from livers of IV-infected mice. (C) Similarly, transcript from FACS-sorted eGFP⁺CD8⁺ T_reg cells was compared to that of eGFP⁻CD8⁺ T_reg cells from day 7 IV-infected Vert-X mice. (D) PD-1 and Tim-3 surface expression along the eGFP profile was separately assessed on Ova Tet⁺ and Ova Tet⁻CD8⁺ T_reg cells (n = 3 per group). (E) Representative in vitro suppression assays from bulk CD8⁺ T_reg cells and FACS-sorted eGFP⁺CD8⁺ T_reg cells or eGFP⁻CD8⁺ T_reg cells from day 7 Ad-Ova IV-infected C57BL/6 and Vert-X mice, respectively, cocultured with CFSE-labeled naïve Thy1.1⁺CD8⁺ OT-I T cells at a 1:1 ratio are depicted (n = 3 per group). (F) Day 3 percent suppression by day 7 Ad-Ova liver-primed CD8⁺ T_reg cells during coculture with CFSE-labeled naïve Thy1.1⁺CD8⁺ OT-I T cells at a 1:1 ratio and SIINKFEKL-pulsed BMDCs are displayed. Select wells also contained anti-IL-10R Ab in media during culture (n = 3 per group). Numbers in histograms and scatter plots represent percentages.