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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Exp Eye Res. 2014 Jan 24;120:61–70. doi: 10.1016/j.exer.2014.01.004

Figure 4.

Figure 4

HRT-RCM highly reflective small cells stained positively for Ly-6G, a PMN marker. (a) HRT image of infiltrating highly reflective bodies (light spots) 8 hours after epithelial injury. Immediately after HRT imaging the eye was removed and labeled with a primary antibody against Ly-6G conjugated with FITC. (b) Fluorescence microscopy of the same corneal region as (a). Comparison of the two images confirms the identity of the cells imaged with HRT-RCM to be Ly-6G+ PMNs. There is a very slight shift in cell positions due to the whole-mount process but out of 51 cells identified in (a), only one could not be correspondingly identified in (b).

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