Figure 7.
Immunolabeling of PMNs in the parawound region of the cornea. Eight hours after anti-integrin antibody blockade, excised corneas were immunolabeled with FITC (green) conjugated secondary antibody. PMNs fluoresced green and their polymorphic nuclei fluoresced blue (DAPI). Topical application of non-immune IgG control antibody did not bind to the PMNs as evidenced by a lack of green staining (upper left panel; open arrowhead). Keratocytes remained unlabeled by secondary antibody but their oval-shaped nuclei were visible after DAPI staining (white arrowheads).