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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Bioorg Med Chem Lett. 2014 Jan 11;24(4):1094–1097. doi: 10.1016/j.bmcl.2014.01.010

Figure 3.

Figure 3

A: Inhibitory activity of selected compounds in an endpoint biochemical assay. Compounds were incubated with SUMO1, E1, E2 enzymes, a fluorescent substrate, and ATP in an appropriate buffer and quenched with EDTA after 90 min. Conversion was quantified by ratiometric peak height in a microfluidic electrophoretic mobility shift assay using a Perkin Elmer EZ Reader II. Values represent the mean of three replicates. B: Evaluation of selected compounds in kinetic biochemical sumoylation assays. Compounds were incubated with SUMO1, E1, E2 enzymes, a fluorescent substrate, and ATP in an appropriate buffer and monitored over the course of 110 minutes. Conversion was quantified by ratiometric peak height in a microfluidic electrophoretic mobility shift assay using a Perkin Elmer EZ Reader II.