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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Dev Biol. 2013 Dec 12;386(1):123–134. doi: 10.1016/j.ydbio.2013.11.020

Figure 1. FGF dependent control of brain asymmetry is uncoupled from earlier LR patterning.

Figure 1

(A–D) Dorsal view of 22–24 somite embryos. L=left, R=right. (A–B) Cyclops is normally expressed in left diencephalon (A; DMSO controls) however when FGF signaling is inhibited, expression becomes bilateral (B; yellow arrowhead. (C–D) Normal left-sided expression of lefty2 within the prospective heart field in the LPM in both DMSO control (C) and SU5402 treated (D) embryos (white arrows). Note that SU5402 was suspended in DMSO in all experiments. Yellow arrowhead indicates bilateral expression of lefty1 in SU5402 treated embryos (D) compared with normal left-sided expression in DMSO treated embryos (C). (E, F) Dorsal view of 18–20 somite embryos showing spaw expression in the LPM. Normal left-sided expression of spaw in the left LPM of SU5402 (F) compared to the DMSO control embryo (E). (G, H) Images of the head dissected from 20 somite embryos showing erm expression (anterior is up). Erm expression is down-regulated in SU5402 treated embryos (H, n=28) in the dorsal diencephalon (red bracket) in comparison to a DMSO control embryo (G, white bracket, n=32). (I) Histogram showing the percentages of embryos displaying normal (left-sided), reversed (right-sided), absent and bilateral expression patterns of cyclops (brain), lefty1 (brain), lefty2 (heart field), spaw (LPM) in SU5402-treated embryos and DMSO-treated embryos. (J) Line graph showing a timeline of SU5402 and DMSO treatments affecting lefty expression in the brain (See Supplemental Figure 1 for all expression classes). 70% epiboly-Tailbud: DMSO n= 29, SU5402=42. 8–24 SS: DMSO n=53, SU5402 n=60. 10–24 SS: DMSO n=49, SU5402 n=57. 12–24 SS: DMSO n=45, SU5402 n=28. 14–24 SS: DMSO n=53, SU5402 n=48. 16–24 SS: DMSO n=64, SU5402 n=37.