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. Author manuscript; available in PMC: 2014 Apr 24.
Published in final edited form as: Nature. 2013 Oct 6;502(7472):567–570. doi: 10.1038/nature12571

Extended Data Figure 9. Distribution of AP-2 and microtubules in MDA-MB-231 cells migrating in a 3D collagen matrix.

Extended Data Figure 9

a-b, MDA-MB-231 cells migrating away the multicellular spheroid at T2 (see Extended Data Methods) were fixed and stained for immunofluorescence microscopy with antibodies against α-adaptin (red) and K40-tubulin (green, panel a) or α-tubulin (green, panel b); the DNA was stained with DAPI (blue; upper panel). Scale bar, 10 μm. The lower panel shows the fluorescence intensity distribution along the long cell axis. Thirteen or ten cells from two independent experiments were analysed, respectively, and the data are expressed as the average fluorescence intensity ± s.e.m. Arrows indicate the apparent direction of migration. c, MDA-MB-231 cells treated with indicated siRNAs and escaping from a spheroid at T2 were fixed in paraformaldehyde and stained for K40-acetylated tubulin. Scale bar, 10 μm. Arrows indicate the apparent direction of migration.