Skip to main content
. Author manuscript; available in PMC: 2014 Apr 24.
Published in final edited form as: Nature. 2013 Oct 6;502(7472):567–570. doi: 10.1038/nature12571

Extended Data Figure 10. α-adaptin- or αTAT1-depletion inhibits persistent cell migration and invasion.

Extended Data Figure 10

a-b, MDA-MB-231 cells treated with non-targeting (NT), α-adaptin, αTAT1 or CHC-specific siRNAs were plated on glass-bottom dishes and imaged by phase-contrast microscopy every 5 min for 8 hours. Cells were manually tracked using Metamorph software and cell velocity (a) and persistent migration index (b) were calculated for each cell and represented as box plots. Error bars indicate mean ± s.e.m (from 150 to 180 cells per conditions from three independent experiments, * P<0.05). c, Multicellular spheroids of MDA-MB-231 cells treated with NT, αTAT1- or AP-2-specific siRNAs, as indicated, were embedded in a 3D collagen matrix and fixed immediately (T0) or 2 days later (T2) then stained with phalloidin. Scale bar, 300 μm.