a, MDA-MB-231 cells treated with non-targeting siRNAs (NT) or with α-adaptin or μ2-adaptin specific siRNAs were stained for α-adaptin and K40 acetyl-tubulin. Scale bar, 10 μm. b, MDA-MB-231 cells were treated with the indicated siRNA and the cell lysates were analysed by western blotting with using the indicated antibodies. c, Quantification of the pixel density of bands detected by the anti-K40 acetyl antibody in (b) expressed in percentage of siNT ± s.e.m (normalized to total tubulin levels, * P<0.001 compared to siNT).