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. Author manuscript; available in PMC: 2014 Apr 24.
Published in final edited form as: Nature. 2013 Oct 6;502(7472):567–570. doi: 10.1038/nature12571

Extended Data Figure 3. Effects of AP-2 knockdown on microtubule post-translational modifications in MDA-MB-231 cells.

Extended Data Figure 3

a, MDA-MB-231 cells treated with non-targeting siRNAs (NT) or with α-adaptin or μ2-adaptin specific siRNAs were stained for α-adaptin and K40 acetyl-tubulin. Scale bar, 10 μm. b, MDA-MB-231 cells were treated with the indicated siRNA and the cell lysates were analysed by western blotting with using the indicated antibodies. c, Quantification of the pixel density of bands detected by the anti-K40 acetyl antibody in (b) expressed in percentage of siNT ± s.e.m (normalized to total tubulin levels, * P<0.001 compared to siNT).